Internal ribosome entry site-based vectors for combined gene …
网页2015年2月2日 · In this context, internal ribosome entry site (IRES)-based vectors provide a powerful system to co-express several therapeutic genes from the same transcription unit. IRESs are translational enhancers, exhibiting tissue-specificity, and activated by stress.
pcDNA3.1(+)IRES GFP - Addgene
网页Plasmid pcDNA3.1 (+)IRES GFP from Dr. Kathleen_L Collins's lab contains the insert IRES GFP and is published in J Immunol. 2008 Jun 15;180 (12):7804-17. This plasmid is available through Addgene.
网页Cell Biolabs’ pMCs-IRES-GFP retroviral vector (also known as pMCs-IG) includes hybrid LTRs containing elements from both MMLV and PCMV, and it’s capable of expressing genes in both EC and ES cells.
pCMV6-AC-IRES-GFP Sequence and Map - SnapGene
网页PrecisionShuttle™ mammalian expression vector with a TurboGFP marker, for fusing an ORF to C-terminal c-Myc and FLAG® tags.
IRES-mediated cap-independent translation, a path leading to …
网页2019年9月3日 · Following this idea, a synthetic IRES containing binding sites for tissue- or cell type-specific ITAFs may be used to achieve tissue-specific protein production through IRES-dependent translation.
pCIG3 (pCMV-IRES-GFP version 3) - Addgene
网页This vector differs from pCIG1 in the following manners: - Different IRES (derived from Clontech pIRES-GFP), which is easier to mutagenize, PCR, and sanger sequence. - …
Analyses of Gnai3-iresGFP reporter mice reveal unknown Gαi3 …
网页2021年7月12日 · An internal ribosomal entry site (IRES) was inserted behind the stop-codon of the Gnai3 gene to initiate simultaneous translation of the GFP cDNA together with Gαi3.
Developing a Secretory AcGFP1-based IRES Expression System for ...
网页In the mammalian expression vector, the DNA encoding the recombinant and AcGFP1 proteins are linked by an IRES, making them transcribed into the same mRNA but translated independently.
In vitro and in vivo comparison of viral and cellular internal ribosome ...
网页2011年3月3日 · The in vitro screening in human and mouse fibroblast and hepatocarcinoma cells revealed that the vascular endothelial growth factor and type 1 collagen-inducible protein (VCIP) IRES was the only...
Developing a secretory AcGFP1-based IRES expression system for ...
网页2022年4月1日 · Highlights • We developed a mammalian protein expression vector using secreted GFP to measure the recombinant gene expression. • GFP is expressed under IRES (internal ribosome entry site) sequence. • Recombinant protein expressions are correlated with GFP fluorescence intensities. •